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bsa i site  (New England Biolabs)


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    Structured Review

    New England Biolabs bsa i site
    Bsa I Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+i+site/BsaI/pmc12972897-117-43-53
    Average 97 stars, based on 2186 article reviews
    bsa i site - by Bioz Stars, 2026-09
    97/100 stars

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    Biosynthesis of monoterpene indole alkaloids. (A) Production of strictosidine from tryptamine and secologanin by the action of Catharanthus roseus strictosidine synthase (CrSTR). (B) Biosynthesis of stemmadenine acetate, an intermediate of the pathway for the production of the two anticancer compounds vinblastine and vincristine. The production of stemmadenine acetate involves 6 enzymes from strictosidine: CrSGD, CrGS, CrGO, <t>CrReDOX1,</t> CrReDOX2, and CrSAT. Akuammicine is a spontaneous degradation product of preakuammicine. (C) Biosynthesis of the antipsychotic molecule alstonine in Catharanthus roseus. The production of alstonine involves 3 enzymes from strictosidine: CrSGD, CrTHAS, CrAS.
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    Biosynthesis of monoterpene indole alkaloids. (A) Production of strictosidine from tryptamine and secologanin by the action of Catharanthus roseus strictosidine synthase (CrSTR). (B) Biosynthesis of stemmadenine acetate, an intermediate of the pathway for the production of the two anticancer compounds vinblastine and vincristine. The production of stemmadenine acetate involves 6 enzymes from strictosidine: CrSGD, CrGS, CrGO, CrReDOX1, CrReDOX2, and CrSAT. Akuammicine is a spontaneous degradation product of preakuammicine. (C) Biosynthesis of the antipsychotic molecule alstonine in Catharanthus roseus. The production of alstonine involves 3 enzymes from strictosidine: CrSGD, CrTHAS, CrAS.

    Journal: Frontiers in Plant Science

    Article Title: Directed Biosynthesis of New to Nature Alkaloids in a Heterologous Nicotiana benthamiana Expression Host

    doi: 10.3389/fpls.2022.919443

    Figure Lengend Snippet: Biosynthesis of monoterpene indole alkaloids. (A) Production of strictosidine from tryptamine and secologanin by the action of Catharanthus roseus strictosidine synthase (CrSTR). (B) Biosynthesis of stemmadenine acetate, an intermediate of the pathway for the production of the two anticancer compounds vinblastine and vincristine. The production of stemmadenine acetate involves 6 enzymes from strictosidine: CrSGD, CrGS, CrGO, CrReDOX1, CrReDOX2, and CrSAT. Akuammicine is a spontaneous degradation product of preakuammicine. (C) Biosynthesis of the antipsychotic molecule alstonine in Catharanthus roseus. The production of alstonine involves 3 enzymes from strictosidine: CrSGD, CrTHAS, CrAS.

    Article Snippet: Synthetic genes CrSGD, CrGS, CrGO, CrRedOx1, CrRedOx2 and CrSAT domesticated for the Bsa I site ( ) were purchased from Twist Bioscience .

    Techniques:

    Production of alstonine and stemmadenine analogs from strictosidine analogs in N. benthamiana following the co-infiltration of the three genes involved in the alstonine pathway (CrSGD, CrTHAS, CrAS) or the six genes involved in the stemmadenine pathway (CrSGD, CrGS, CrGO, CrReDOX1, CrReDOX2, CrSAT).

    Journal: Frontiers in Plant Science

    Article Title: Directed Biosynthesis of New to Nature Alkaloids in a Heterologous Nicotiana benthamiana Expression Host

    doi: 10.3389/fpls.2022.919443

    Figure Lengend Snippet: Production of alstonine and stemmadenine analogs from strictosidine analogs in N. benthamiana following the co-infiltration of the three genes involved in the alstonine pathway (CrSGD, CrTHAS, CrAS) or the six genes involved in the stemmadenine pathway (CrSGD, CrGS, CrGO, CrReDOX1, CrReDOX2, CrSAT).

    Article Snippet: Synthetic genes CrSGD, CrGS, CrGO, CrRedOx1, CrRedOx2 and CrSAT domesticated for the Bsa I site ( ) were purchased from Twist Bioscience .

    Techniques: